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Depletion of Iba57p results in diminished de novo Fe/S cluster formation on homoaconitase (Lys4p). The indicated strains carrying p426LYS4-HA were grown in iron-poor SC medium containing the indicated carbon source. Cells were radiolabeled and Lys4p immunoprecipitated with anti-HA (Santa Cruz) immunobeads as described in Fig. 2. Lys4-HA was detected by immunostaining with anti-HA. Por1p was used as a loading control.
