FIG. 1.
Postnatal lethality and cortical dysplasia in nestin-Cre; Gna12−/−; Gna13flox/flox mice. (A) Western blot analysis of Gα12, Gα13, and Gαq/Gα11 expression in brain extracts prepared from wild-type (WT) and nestin-Cre; Gna12−/−; Gna13flox/flox (KO) mice. (B) Postnatal survival of nestin-Cre; Gna12−/−; Gna13flox/flox, nestin-Cre; Gna12−/+; Gna13flox/flox, nestin-Cre; Gna12−/−; Gna13+/flox, and Gna12−/−; Gna13flox/flox mice; the total number of analyzed animals was 54, 51, 38, and 29, respectively. (C) Sagittal section through the cerebellum of wild type (WT) and nestin-Cre; Gna12−/−; Gna13flox/flox (KO) animals at P21 stained with an anti-NeuN antibody. Cortical malformations in the knockout cerebella were restricted to the rostral part of the vermis. (D) Frontal sections of the cerebral cortex from wild type (WT) and nestin-Cre; Gna12−/−; Gna13flox/flox (KO) P21 brains stained with an anti-NeuN antibody. Scale bars are 250 μm (panel C) and 125 μm (panel D).