FIG. 3.

Specificity of the association between the VE-cad-based complex and the endothelial annexins. Anti-VE-cad (A), anti-A1 (B), and anti-A5 (C) IPs were performed on confluent HUVECs and were resolved on 12% Tris-glycine gels. Anti-VE-cad IPs were immunoblotted for VE-cad and A1, A2, and A5. Anti-A1 and anti-A5 IPs were probed for VE-cad and either A1 or A5, respectively. For comparison, aliquots of whole-cell lysates (Lys) were analyzed in parallel. Molecular mass markers (kDa) are given at the left margin of each blot.