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Calculation of affinities of heparin for collagenous peptides and type I collagen. From ACE gel electrophoretograms, retardation coefficients (R) for heparin migration were determined and plotted against protein concentration. Smooth curves represent nonlinear least-squares fits to the equation R = R∞/{1 + (Kd/[protein])2}. THP sequences and apparent Kd values for heparin binding derived from ACE gels are shown in Tables 1 and 2.
