Table 1.
Collagenous peptides used in this study
| Peptide | Amino acid sequence |
|---|---|
| α1(I)82–93 THP | [(GPP*)6-GLPGMKGHRGFS-Ahx]3-KKYG |
| α1(I)82–93X THP | [(GPP*)6-GFLGRSGMKGDH-Ahx]3-KKYG |
| α2(I)85–94 THP | [(GPP*)6-GFKGIRGHSG-Ahx]3-KKYG |
| α2(I)85–94X THP | [(GPP*)6-GRSGFKGIH-Ahx]3-KKYG |
| α1(I)787–796 THP | [(GPP*)6-GQRGERGFPG-Ahx]3-KKYG |
| α1(I)925–937 THP | [(GPP*)6-GDRGIKGHRGFSG-Ahx]3-KKYG |
| (GPP*)8 | [(GPP*)8-Ahx]3-KKYG |
| α1(I)82–94X SSP | GFLGRSGMKGPHG |
| α2(I)85–94 SSP | GFKGIRGHSG |
| α2(I)85–94X SSP | GRSGFKGIHG |
| α1(I)787–796 SSP | GQRGERGFPG |
| α1(I)925–937 SSP | GDRGIKGHRGFSG |
THPS carry sequences corresponding to amino acid positions from the human sequences (49, 50) as numbered from the beginning of the type I collagen triple helix: α1(I)82–93 THP and α2(I)85–94 THP, which include the proposed N-terminal heparin-binding domain of type I collagen (6) or the same sequences in scrambled order as denoted by an X following the amino acid position designation; α1(I)925–937 THP comprises a C-terminal site homologous to the proposed heparin-binding site. Scrambled sequences of these sites are also shown. Other THPs include one containing the slightly basic sequence at positions α1(I)787–796 THP and another carrying eight repeats of the collagenous triplet Gly-Pro-Hyp, (GPP*)8. SSPs carrying native or scrambled sequences selected from those carried by the THPs are denoted by SSP; those carrying scrambled sequences are denoted by X SSP.