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Northern blot analysis of sae transcripts in COL (wild type [wt]) and its isogenic sae and rot mutants. The probe for sae was a 402-bp fragment containing ORF4 of sae, which yielded two transcripts (T3 and T4) driven only by the sae P3 promoter. RNA was harvested from cells grown to an OD650 of 1.7. The RNA gels below show equivalent sample loadings.
