TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Comment | Reference |
|---|---|---|
| Strains | ||
| E. coli XL-1 Blue | General-purpose host strain for cloning | 14 |
| S. aureus | ||
| RN4220 | Mutant strain of 8325-4 that accepts foreign DNA | 18 |
| COL | Methicillin-resistant laboratory strain | 21 |
| ALC4481 | COL sae single-deletion mutant | This study |
| ALC4909 | COL rot single-deletion mutant | This study |
| ALC4988 | COL sae rot double-deletion mutant | This study |
| ALC5245 | COL sae rot sarT triple-deletion mutant | This study |
| ALC3227 | COL with pALC1740 | This study |
| ALC5097 | ALC4481 with pALC1740 | This study |
| ALC5098 | ALC4909 with pALC1740 | This study |
| ALC5099 | ALC4988 with pALC1740 | This study |
| ALC5256 | ALC5245 with pALC1740 | This study |
| ALC5018 | COL with pALC4989 | This study |
| ALC5019 | COL with pALC4990 | This study |
| ALC5020 | COL with pALC4991 | This study |
| ALC5370 | ALC4481with pALC4991 | This study |
| ALC5371 | ALC4909 with pALC4991 | This study |
| ALC5373 | ALC4988 with pALC4991 | This study |
| ALC5376 | ALC5245 with pALC4991 | This study |
| Plasmids | ||
| pALC1484 | Modified pALC236 shuttle vector with a promoterless gfpuvr reporter gene preceded by an S. aureus ribosome binding site | 11 |
| pALC1740 | pALC1484 with the hla promoter | 15 |
| pALC4989 | pALC1484 with the sae P1 promoter fused with the gfpuvr reporter gene at the EcoRI and XbaI sites | This study |
| pALC4990 | pALC1484 with the sae P2 promoter fused with the gfpuvr reporter gene at the EcoRI and XbaI sites | This study |
| pALC4991 | pALC1484 with the sae P3 promoter fused with the gfpuvr reporter gene at the EcoRI and XbaI sites | This study |
| pMAD | Vector for allelic replacement in gram-positive bacteria | 1 |