TABLE 2.
Primers used in this study
| Use | Primera |
|---|---|
| sae deletion | 5′GTCAGGATTCGTACGGATACCACTATAGAT3′ |
| 5′TCCGATTTATTTATAAAATAAAATGCAAAGACTAAAAAGAAGCTC3′ | |
| 5′CATTTTATTTTATAAATAAATCGGACTATTTTTTCACCTCTGTTCTTACGA3′ | |
| 5′GATCCCATGGTCCAGATTTATACGTCTACCTAACA3′ | |
| rot deletion | 5′GATCGGATCCCACGAGGTTCACAATGAGC3′ |
| 5′CCCAACAATCCCAAAACTTGTATGTGCT3′ | |
| 5′ATACAAGTTTTGGGATTGTTGGGGTTTAATAGCATAAAAAGAGGT3′ | |
| 5′GATCCCATGGTGACTCAAGAAGAGTACACAAAC3′ | |
| sarT deletion | 5′GTCAGGATTCATCCTTTCATCTGCAAGGGATCGT3′ |
| 5′CACCAAGATATTAAAATCTCGCAAATCATTCATCAAGTCTTC3′ | |
| 5′GAAGACTTGATGAATGATTTGCGAGATTTTAATATCTTGGTC3′ | |
| 5′GTCAGAATTCATGGTTATTTGCCACTCTAAC3′ | |
| sae P1 promoter-gfp fusion | 5′GTCAGAATTCTCGCAATGGTTGACTACGAT3′ |
| 5′GTCATCTAGAATTTATTGTGTGTAATTTATATAAACA3′ | |
| sae P2 promoter-gfp fusion | 5′GTCAGAATTCTTAGTACCAGTCATCGCTAAC3′ |
| 5′GTCATCTAGACTTACGACCTCTAAAGTAATTAATGAT3′ | |
| sae P3 promoter-gfp fusion | 5′GTCAGAATTCTTATTGTGGCAAAAGGTTTATAAA3′ |
| 5′GTCATCTAGACAATTTGATAAGTTAAGTTTAAAAT3′ |
a
Restriction sites are in bold and underlined.