FIG. 8.

Competitive index infections of ΔagfA strain and strain ATCC 14028 in C57BL/6 mice. Groups of mice were infected with a mixed inoculum of ΔagfA strain and strain ATCC 14028 cells that were grown in nutrient-rich conditions at 37°C (NR) or nutrient-limiting conditions at 28°C (NL). The total numbers of colonizing Salmonella (A) and CI for the ΔagfA strain (B) were determined in the spleen, MLN, colon, and small intestine (Sm Int.). Each data point represents one animal, and horizontal bars indicate means. The broken line in panel A represents the limit of detection (10 CFU), and the broken line in panel B represents a CI value of 1, which would result if there was no competitive difference between the ΔagfA strain and strain ATCC 14028. P values are listed above CI results from each organ, The asterisk represents statistical significance (P < 0.05).