FIG. 9.

Bioluminescence imaging of Salmonella rdar morphotype gene expression in mice infected by oral gavage. (A) In vivo bioluminescence of the gastrointestinal tracts and organs of C57BL/6 mice following infection with strain ATCC 14028 agfD, agfB, yihU, sig70_16 (σ⁷⁰), and sig38H4 (RpoS) luciferase reporter strains. Images show the relative signal intensity visualized at a given anatomical location within tissue (small intestine [Sm Int], mesenteric lymph nodes[MLN], spleen [Sp], liver [Li], and colon [Co]). In the color bars displayed on the right of each image, red corresponds to the highest signal intensity and blue corresponds to the lowest signal intensity in units of light measurement (photons/second/cm²/seradian). Representative images are shown from 3 (yihU and RpoS) or 13 (agfD, agfB, and σ⁷⁰) mice. (B) Bioluminescent images of fecal pellets from mice infected with strain ATCC 14028 reporter strains. Pellets were collected between 60 h and 84 h postinfection. The scale of signal intensity for each image is located on the right.