TABLE 2.
Hepta-serotype HCR vaccine protects against challenge by the seven serotypes of BoNTa
| Serotype | No. of surviving mice/total no. challenged with serotype at:
|
|
|---|---|---|
| 1,000 LD50 | 10,000 LD50 | |
| A | 5/5 | 5/5 |
| B | 5/5 | 5/5 |
| C | 4/5 | 4/4 |
| D | 5/5 | 5/5 |
| E | 5/5 | 5/5 |
| F | 5/5 | 5/5 |
| G | 5/5 | 5/5 |
Mice were immunized as follows: day 0, 1 μg of each HCR (serotypes A to G) with Alhydrogel; day 14, 1 μg of each HCR with Alhydrogel; day 28, 1 μg of each HCR with Alhydrogel; day 42, 1 μg of each HCR alone. On day 52, mice were challenged individually with 1,000 LD50 of the indicated serotype of BoNT. After 3 days, survival was scored, and survivors were challenged with 10,000 LD50 of the indicated BoNT serotype and scored for survival at 4 days. Data presented are representative of several vaccine trial experiments performed with this immunogen. Control experiments showed that five of five mice vaccinated with the adjuvant alone did not survive challenge with 100 LD50 of BoNT/A.