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. 2007 Dec 17;76(3):959–966. doi: 10.1128/IAI.01455-07

FIG. 1.

FIG. 1.

Inhibitory effect of monoclonal antibody 10D11 on E. histolytica phagocytosis of apoptotic lymphocytes. Antibody-treated E. histolytica trophozoites (4°C, 20 min) were incubated with fluorescent apoptotic Jurkat T cells (E. histolytica-to-Jurkat cell ratio of 1:2, 37°C, 10 min) in the presence of 110 mM d-galactose to inhibit the Gal/GalNAc lectin. Phagocytosis was assayed using flow cytometry. (A) Representative fluorescence-activated cell sorter histograms showing amebic fluorescence (20-μg/ml antibody dose). The M1 gate indicates phagocytic amebae. (B) Dose effect of 10D11Ab on E. histolytica phagocytosis (mean percentage of PBS effect with standard deviation [SD]) (n = 6). Squares, 10D11Ab; diamonds, 5E12Ab (anti-E. histolytica membrane IgG1 control). Asterisks indicate P values of <0.0003 compared to the control. (C) Confocal microscopy verifying reduced amebic phagocytosis of apoptotic lymphocytes following treatment with 10D11Ab. Original magnification, ×200.