TABLE 1.
CD4+ T-cell epitopes recognized by immunized animals as determined by proliferation assays
| Protein | Animal | Stimulation indexa
|
|||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| RAP-1 peptideb | F2-1c | F2-2 | F2-3 | F2-4 | F2-5 | F3-1 | F3-2 | F3-3 | F3-4 | F3-5 | F3-6 | ||
| Native MSP1 | 4841 | 1 | 1 | 13 | 7 | 1 | 1 | 1 | 4 | 9 | 1 | 1 | 2 |
| 4862 | 1 | 1 | 25 | 2 | 11 | 2 | 2 | 1 | 22 | 1 | 2 | 1 | |
| 4882 | 1 | 1 | 73 | 2 | 15 | 1 | 1 | 8 | 11 | 1 | 1 | 1 | |
| 4906 | 1 | 1 | 14 | 3 | 4 | 1 | 2 | 1 | 17 | 1 | 1 | 1 | |
| Linked | 4859 | 1 | 2 | 6 | 1 | 4 | 2 | 3 | 1 | 15 | 8 | 1 | 1 |
| 4904 | 4 | 1 | 27 | 11 | 9 | 2 | 34 | 1 | 116 | 1 | 17 | 2 | |
| 4885 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | |
| 4919 | 1 | 1 | 3 | 1 | 4 | 1 | 2 | 1 | 26 | 1 | 1 | 1 | |
| Unlinked | 4911 | 1 | 36 | 33 | 10 | 33 | 2 | 3 | 19 | 65 | 35 | 1 | 4 |
| 4916 | 4 | 17 | 61 | 34 | 43 | 3 | 38 | 6 | 63 | 9 | 37 | 2 | |
| 4914 | 1 | 2 | 8 | 2 | 6 | 2 | 8 | 1 | 5 | 6 | 1 | 1 | |
| 4932 | 1 | 4 | 8 | 1 | 4 | 1 | 1 | 1 | 14 | 1 | 1 | 1 | |
a
PBMC were obtained 3 weeks after the fourth immunization and used in a 6-day proliferation assay with 1 μg/ml of the indicated peptides. Stimulation indices were calculated by dividing the mean cpm of PBMC cultured with antigen by the mean cpm of PBMC cultured with medium. The data were analyzed by ANOVA using SAS, version 9.1. Significant responses are indicated by bold type.
b
RAP-1 peptide is a negative control peptide derived from B. bovis RAP-1.
c
F2-1 to F2-5 and F3-1 to F3-6 are peptides in the F2-F3 region of A. marginale MSP1a (6).