FIG. 7.

Uptake of N. meningitidis by granulocytes. GFP-expressing C311 cells were grown, fixed in PFA, washed, and opsonized with CRP (cross-hatched bars) or incubated with PBS (stippled bars) before incubation with granulocytes adhered to plastic chamber slides. Slides were washed, fixed, and stained with propidium iodide, and at least 100 cells from 10 randomly selected fields of view were counted for enumeration of the percentage of granulocytes associated with bacteria (a) and the mean number of adherent or intracellular bacteria per granulocyte (b). Data are means from three replicate experiments plus standard deviations (*, P < 0.05).