TABLE 1.
Oligonucleotide primers used in this study
| Application | Primer | Sequencea |
|---|---|---|
| Sequencing | RALP-F | GCGGGTACCCTTTATAATATAGGAATATAAAAATG |
| RALP-R | GCGCCCTAGGAAAAAGCTCAAATAAGAC | |
| MSP1-F | AAGCTTTAGAAGATGCAGTATTGAC | |
| MSP1-R | AATCATTAA TTTCTTCATATCCATC | |
| Transfection | GFP-F | GCGCGGGTACCAGTAAAGGAGAAGAACTT |
| GFP-R | GCGCCTCGAGGGCCTAGGTTTGTATCGTTCATCCAT | |
| RALP5′-F | GCGCGGTACCGGAA TATAAAAATG | |
| RALP5′-R | GCGCGGTACCTGCCACATTAGG | |
| RALP3′-F | GCGCCCTAGGTCACACGACATATCA | |
| RALP3′-R | GCGCCTCGAGTTAAAAAAGC TC | |
| RALP-TY1-R | GCGCCTCGAGTCAGTCAAGTGGATCCTGGTTAGTATGGACCTCAAAAAGCTCAAATAAGACTTTG | |
| RALP5′KO-F | GCGCGGTACCGGAA TATAAAAATG | |
| RALP5′KO-R | GCGCGGTACCTGCCACATTAGG | |
| RALP3′KO-F | GCGCGAATTCTAAGACCACAAAAATGAGATGACCAC | |
| RALP3′KO-R | GCGCCCATGGTTACATCTATATCAGAATCGTTTATGTC | |
| Expression | RALP-N-F | GCGCGGATCCCCACTTCAACGTGGCGCATC |
| RALP-N-R | GCGCCTCGAGTTAATCAACGTTCACATTCATTTG | |
| RALP-C-F | GCGCGGATCCGGACAAGGAGAATTACTACAATC | |
| RALP-C-R | GCGCCTCGAGTTAGTTATCTTTTTGTTTGTTTCCC | |
| ASP-F | GCGCGGATCCGGACAAGGAGAATTACTACAATC | |
| ASP-R | GCGCCTCGAGTTAGTTATCTTTTTGTTTGTTTCCC |
a
Restriction endonuclease sites are underlined and in italics, and stop codons are in boldface.