FIG. 2.
Bim−/− mice have increased L. major-specific T-cell responses. Groups of either Bim+/+ or Bim−/− mice (six mice/group) were infected intradermally in the left ear with 3 × 103 L. major promastigotes. At 15 weeks the T-cell responses in the infected ear and draining LNs were assessed using intracellular cytokine staining and flow cytometry. The graphs show (A) the percentages and (B) the total numbers of L. major-specific CD4+ T cells able to produce IFN-γ; the error bars in panel B indicate the standard errors of the means. An asterisk indicates that there was a significant increase in the total numbers of IFN-γ-producing T cells in Bim−/− mice compared to Bim+/+ mice (P < 0.022 for ears and P < 0.012 for LNs, Student's t test).