FIG. 2.

Characterization of enteric salmonellosis. Mice were orally inoculated with 10⁸ CFU S. enterica serovar Typhimurium or buffer alone and then sacrificed after either 3 or 7 days. The mouse terminal ileum was removed and fixed in Carnoy's fixative. Hematoxylin and eosin staining was performed on terminal ileum sections from control (a) and Salmonella-infected (b and c) mice at 7 days postinfection. P, Peyer's patch; C, crypt epithelium; LP, lamina propria. Arrows indicate the locations of neutrophils. (d) The ceca of control and infected mice were removed and weighed for comparison at both 3 and 7 days postinfection. Black squares represent uninfected control mice. Black triangles represent Salmonella-infected mice. Bacterial genomic DNA was isolated from the DSI, ceca, and LI of control and infected mice at 3 (e) and 7 (f) days postinoculation and analyzed by qPCR for total bacteria. White bars represent uninfected controls. Black bars represent Salmonella-infected mice. *, P < 0.05 (Student's t test).