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Mutation of bscN in Bordetella spp. abolishes Bsp22 production but does not affect secretion of the virulence factor FHA. Proteins from culture supernatants of WT and ΔbscN B. pertussis (Bp) 12743 and B. bronchiseptica (Bb) RB50 or B. pertussis Tohama I (Toh-I) were examined for the presence of Bsp22 and FHA by Western blotting.
