TABLE 1.
Identification of secreted TTSS substrates and other virulence factors from Bordetella spp. by MALDI-TOF mass spectrometrya
| Mascot accession no. | Identified protein | Result for:
|
||||
|---|---|---|---|---|---|---|
| B. pertussis Toh I | B. pertussis 12743 | ΔbscN B. pertussis 12743 | B. bronchiseptica RB50 | ΔbscN B. bronchiseptica RB50 | ||
| BP1879/BB2993 | FHA | + (141) | + (8,869) | + (3,776) | + (567) | + (434) |
| BP0760/BB0324 | Bifunctional hemolysin-adenylate cyclase toxin (CyaA) | + (116) | + (2,423) | + (1,074) | + (2,551) | + (2,571) |
| BP1054/BB1366 | Pertactin (Prn) | + (111) | + (2,066) | + (759) | + (450) | + (350) |
| BP3494/BB0961 | Serum resistance protein (BrkA) | + (234) | + (2,261) | + (1,893) | − | − |
| BP1201BB3291 | Tracheal colonization factor (TcfA) | + (412) | + (1,530) | + (3,117) | − | − |
| BP2256/BB1617 | Putative secreted protein (Bsp22) | − | + (470) | − | + (500) | − |
| BP2253/BB1620 | Putative outer protein D (BopD) | − | + (781) | − | + (193) | − |
| BP2257/BB1616 | Putative outer protein N (BopN) | − | + (204) | − | − | − |
| BP0500/BB4228 | Hypothetical protein (BteA/BopC) | − | − | − | + (67) | − |
a
Quantitative MALDI-TOF tandem mass spectrometry analysis was carried out on extracted proteins from culture supernatant of B. pertussis Tohama I (Toh I), B. pertussis 12743, ΔbscN B. pertussis 12743, B. bronchiseptica RB50, and ΔbscN B. bronchiseptica RB50. Identified peptides were searched against the complete B. bronchiseptica and B. pertussis genome sequences (www.sanger.ac.uk/Projects/B_pertussis/) using the Mascot search engine. Numbers in parentheses represent the individual Mascot ion scores. Individual ion scores >33 indicate identity or extensive homology (P < 0.05).