TABLE 1.
Expt no. | Anesthetic | Time postinfection (h) | Frequency of luminescence
|
||
---|---|---|---|---|---|
In live animals | In situa | On dissection of tissue | |||
1 | Isoflurane | 1 | Not imaged | Not imaged | 2/8 |
Isoflurane | 2 | Not imaged | Not imaged | 6/8 | |
Isoflurane | 4 | Not imaged | Not imaged | 6/8 | |
2 | Isoflurane | 0.5 | Not imaged | Not imaged | 3/4 |
Isoflurane | 1 | Not imaged | Not imaged | 2/4 | |
Isoflurane | 6 | Not imaged | 2/4 | 4/4 | |
Isoflurane | 24 | 0/4 | 2/4 | 3/4 | |
Isoflurane | 48 | 1/4 | 1/3 | 3/3 | |
3 | Ketamine | 1 | Not imaged | Not imaged | 1/3 |
Ketamine | 6 | Not imaged | 2/5 | 4/5 | |
Ketamine | 72 | 1/4 | Not imaged | 4/4 |
Euthanized animals were imaged after the abdomen and thorax were opened to reveal respiratory and digestive tracts and prior to extraction of tissues.