FIG. 1.

Direct binding ELISA to measure binding of 5 nM of recombinant GST-PM1665 to immobilized mammalian ligands found in the extracellular matrix or in plasma. The amount of rGST-PM1665 bound to each ligand was estimated using a standard curve constructed by coating microtiter plate wells with known amounts of rGST-PM1665. The data are presented as the mean ± standard error of the mean of triplicate wells and are representative of three separate experiments. Bars with different letters are significantly different from each other (one-way ANOVA; see the text for P values).