FIG. 11.

(A) Demonstration of the presence of PM1665 protein in P. multocida stationary-phase cells using anti-PM1665 protein antibody and immunogold labeling. Ten-nanometer colloidal-gold labeling is present on P. multocida cells (the gold probes are indicated by arrows), some of which was within the bacterial cell and some of which was located on or near the cell surface. (B) In contrast, antibody purified from preimmune serum did not react with the bacterial cells. (C) Incubation of P. gingivalis cells with anti-PM1665 protein did not result in any labeling. The procedures for these experiments are described in the text.