FIG. 2.

Direct binding ELISA to determine the interaction of 50 nM of rGST-PM1665 with immobilized fibronectin (A) or type I collagen (B). Wells were coated with equimolar concentrations of fibronectin or collagen, and after blocking, doubling dilutions of rGST-PM1665 (closed circles) or rGST (open circles) were added to the wells in quadruplicate. The estimated concentrations of rGST and rGST-PM1665 binding to the fibronectin- or collagen-coated wells are shown as the mean ± standard error of the mean of quadruplicate wells. The data are representative of three such experiments.