Ligand blots showing the binding of rGST-PM1665 to immobilized or soluble fibronectin. (A) rGST-PM1665 was subjected to SDS-PAGE, blotted onto a nitrocellulose membrane, and probed with fibronectin and then with an anti-fibronectin antibody. (B) Fibronectin was subjected to SDS-PAGE, blotted onto nitrocellulose, and incubated first with rGST-PM1665 and then with an anti-GST antibody. Molecular mass markers (in kilodaltons) are indicated on the left. +ve control and −ve control, positive and negative controls, respectively.