FIG. 5.

Inhibition ELISA to determine the capacities of fibronectin and fragments of fibronectin to block the binding of rGST-PM1665 to immobilized human serum fibronectin. The competing ligands (soluble serum fibronectin, superfibronectin [sFn], and the 120-kDa α-chymotryptic fragment of fibronectin) were added at concentrations ranging from 1 nM to 4.6 μM. The results are presented as the mean ± standard error of the mean of quadruplicate wells and are representative of at least three experiments. The values for rGST-PM1665 refer to the binding of the protein to fibronectin-coated wells with no competing ligands. OD₄₉₂, optical density at 492 nm.