FIG. 7.
Ligand blotting showing binding of biotinylated rGST-PM1665 to fibronectin or fragments of fibronectin. (A) SDS-PAGE gel stained with colloidal blue. Lanes: 1, whole fibronectin; 2, Fn III1-2; 3, Fn III4-7; 4, GST-Fn III8-10;, 5, Fn III9-10; 6, GST-Fn III11. (B) Ligand blot of a gel identical to that shown in panel A. The nitrocellulose membrane was incubated with biotinylated rGST-PM1665, followed by incubation with streptavidin-HRP. Recombinant GST-PM1665 bound preferentially to the GST-FnIII8-10 and FnIII9-10 fragments.