TABLE 1.

Primer pairs used in this study

Recombinant GST fusion protein	Size (bp)	No. of amino acids	Primer paira
rGST-PM1665	345	94	5′-GGATCCAATCAGCAAACATGAC-3′
			5′-GAATTCTTAGAGTTGAAGGCGTG-3′
Mature PM1665	345	115	5′-GATCCGAATCAGCAAACATGAC-3′
			5′-GAATTCTTAGAGTTGAAGGCGTG-3′
16S rRNA			5′-AAGTCGTAACAAGGTA-3′
			5′-GGTACTTAGATGTTTCAGTTC-3′
rGST-PM1665 54-115	161-345	54-115	F 5′-CGCGAATTCGTGAATATTAATACAGCCAGC-3′
			R 5′-GAATTCTTAGAGTTGAAGGCGTG-3′
rGST-PM1665 61-115	181-345	61-115	F 5′-CGCGAATTCGCAACTGACTTGCAACAAAAG-3′
			R 5′-GAATTCTTAGAGTTGAAGGCGTG-3′
rGST-PM1665 54-86	161-256	54-86	F 5′-CGCGAATTCGTGAATATTAATACAGCCAGC-3′
			R 5′-CCGCTCGAGATGCTTTTCTCGGTGTTGGAT-3′

^aRestriction sites are underlined.