TABLE 2.
Extracellular host proteins associate with S. aureus in the airway 30 min and/or 6 h postinoculation
| Biological process(es) | Host protein(s) | Level of confidence in IDa at:
|
|
|---|---|---|---|
| 30 min | 6 h | ||
| Coagulation | Alpha-2-antiplasmin | High | Very high |
| Antithrombin III | Very high | ||
| Coagulation factor XIII | Very high | ||
| Fibrinogen | Very high | Very high | |
| Heparin cofactor 2 | Very high | ||
| Hyaluronan binding protein 2 | High | ||
| Kininogen 1 | Very high | Very high | |
| Plasminogen | Very high | ||
| Prothrombin | Very high | ||
| Extracellular matrix | Fibronectin | Very high | |
| Nephronectin | High | ||
| Neutrophil collagenase/matrix metalloproteinase 8 | Very high | ||
| Neutrophil gelatinase-associated lipocalin | Very high | ||
| Vitronectin | High | Very high | |
| Inflammation | Alpha-1-microglobulin/bikunin | High | Very high |
| Calgranulin A/protein S100-A8 | High | ||
| Cathelicidin antimicrobial peptide | Very high | ||
| Chitinase 3-like protein 1 | Very high | High | |
| Chitinase 3-like protein 3 | Very high | Very high | |
| Complement C3 | Very high | Very high | |
| Complement C4b | Very high | ||
| Complement C5 | Very high | Very high | |
| Complement C7 | High | ||
| Complement C8 | Very high | High | |
| Complement C9 | Very high | High | |
| Complement factor B | Very high | ||
| Complement factor H | High | ||
| Granulin/epithelin | High | ||
| Igs (11 identifications)b | Very high | Very high | |
| Long palate, lung, and nasal epithelium carcinoma-associated protein 1 | Very high | ||
| Long palate, lung, and nasal epithelium carcinoma-associated protein 3 | High | ||
| Lysozyme | Very high | Very high | |
| Myeloid bactenecin | Very high | ||
| Palate, lung, and nasal epithelium clone | High | Very high | |
| Serum amyloid A-1 | Very high | ||
| Serum amyloid A-2 | High | ||
| Serum amyloid A-4 | Very high | ||
| Serum amyloid P component | Very high | ||
| Metabolism | Serum paraoxonase/arylesterase 1 | Very high | |
| Proteolysis | Alpha-1-antitrypsins 1-2 | Very high | Very high |
| Alpha-1-antitrypsins 1-4 | Very high | Very high | |
| Alpha-1-antitrypsins 1-5 | Very high | Very high | |
| Alpha-1-antitrypsins 1-6 | Very high | Very high | |
| Alpha-2-macroglobulin | Very high | ||
| Histidine-rich glycoprotein | Very high | ||
| Inter-alpha trypsin inhibitor heavy chain 4 | Very high | ||
| Inter-alpha trypsin inhibitor heavy chain H2 | High | ||
| Murinoglobulin-1 | Very high | ||
| Myeloblastin | High | ||
| Napsin-A | Very high | Very high | |
| Plasma protease C1 inhibitor | High | ||
| Serine proteinase inhibitor B1a | High | High | |
| Serine proteinase inhibitor B6 | Very high | ||
| Serine proteinase inhibitor B6b | Very high | ||
| Serine proteinase inhibitor B9 | Very high | ||
| Serine protease inhibitor A3C | High | ||
| Serine protease inhibitor A3K | Very high | Very high | |
| Serine protease inhibitor A3M | High | Very high | |
| Serine protease inhibitor A3N | Very high | Very high | |
| Whey acidic protein 4-disulfide core domain protein 2 | Very high | Very high | |
| Regulation | α2-HS-glycoprotein/fetuin | Very high | Very high |
| Protein-glutamine gamma-glutamyltransferase 2 | High | ||
| Pulmonary surfactant-associated protein B | Very high | Very high | |
| Pulmonary surfactant-associated protein D | Very high | Very high | |
| Regulation and transport | Apolipoprotein A-I | Very high | Very high |
| Apolipoprotein A-II | High | ||
| Apolipoprotein A-IV | Very high | Very high | |
| Apolipoprotein C-I | High | ||
| Apolipoprotein C-III | High | Very high | |
| Apolipoprotein E | Very high | ||
| Apolipoprotein H/beta-2-glycoprotein I | High | ||
| Signaling | Uteroglobin | Very high | Very high |
| Stress response and regulation | Clusterin | Very high | Very high |
| Transport | Apolipoprotein B | Very high | |
| Ceruloplasmin | Very high | ||
| Haptoglobin | Very high | Very high | |
| Hemopexin | Very high | Very high | |
| Mucin 5B | Very high | ||
| Phospholipid transfer protein | Very high | High | |
| Pulmonary surfactant-associated protein A | Very high | Very high | |
| Serotransferrin | Very high | Very high | |
| Serum albumin | Very high | Very high | |
| Transthyretin | High | ||
| Vitamin D binding protein | Very high | ||
| Transport and metabolism | Apolipoprotein M | High | |
| Unknown | Leubrin | High | |
a
A high level of confidence in the identification (ID) indicates that the protein was confidently identified using one peptide, and a very high level of confidence in the identification indicates that the protein was confidently identified using two or more peptides.
b
A total of 11 different Ig proteins were identified in the analysis (see Table S1 in the supplemental material). The 11 different Igs were combined in this table for simplicity.