FIG. 1.
Effects of LMP7 and MECL-1 on MHC-I cell surface expression (A) and CD4/CD8 ratios (B) in uninfected and L. monocytogenes-infected mice. Splenocytes of age-matched B6 wt, MECL-1−/−, LMP7−/−, and LMP7−/− plus MECL-1−/− mice were stained with anti-CD19-APC, biotin-conjugated anti-H-2Kb, and SAV-PE (uninfected mice; n = 4); anti-CD19-PE, biotin-conjugated anti-H-2Kb, and SAV-APC (infected mice) (A); or anti-CD4-FITC and anti-CD8α-APC antibodies (B) and analyzed by flow cytometry. Mean fluorescence channels of the CD19+ populations (A) and CD4/CD8 T-cell ratios (means plus standard deviations; n = 5 or 6) (B) are depicted.