FIG. 2.

Effects of LMP7 and MECL-1 in Listeria-infected mice. F₂ offspring of B6 wt × LMP7^−/− plus MECL-1^−/− mice were infected with 5 × 10³ rLM-E1 cells intravenously. The spleens were collected at day 7 after infection and analyzed for the presence of LMP7 and/or MECL-1 by immunoblot analysis, using specific rabbit antisera (x axis). (A) Total numbers of splenocytes per spleen (means plus standard deviations). (B and C) Cells were stained with anti-CD8α-APC and anti-CD4-FITC to determine the relative frequencies of CD8 and CD4 T cells, respectively. (D) Cells were incubated with 500 nM synthetic E1B_192-200 for 6 h in the presence of monensin, and the frequencies of E1B_192-200-specific CD8 T cells were determined by staining for CD8 (anti-CD8α-FITC) and intracellular IFN-γ (IFN-γ-PE). Background, detected in samples incubated without peptide, was subtracted.