FIG. 3.

MHC-I cell surface levels and CD4/CD8 T-cell ratios of LMP7^pos plus MECL-1^pos and LMP7^neg plus MECL-1^neg T-cell subsets in mixed BM-chimeric mice. Lethally irradiated LMP7^−/− plus MECL-1^−/− mice were reconstituted with a mixture of BM from B6.SJL (wt; CD45.1^pos) and LMP7^−/− plus MECL-1^−/− (ko; CD45.2^pos) mice and 28 days later were infected with rLM-E1. (A) Splenocytes of mixed BM-chimeric mice were stained at day 28 after BM injection with fluorochrome-conjugated anti-CD45.1, anti-CD45.2, anti-CD19, and biotin-conjugated anti-H-2K^b and SAV-APC to determine the H-2K^b expression levels on the CD45.1 and CD45.2 B-cell subsets. Mean fluorescence channels are depicted (means plus standard deviations [SD]; n = 2). (B) Relative frequencies of CD45.1^pos and CD45.2^pos CD4 and CD8 T cells in the spleens of mixed BM-chimeric mice were determined at day 28 after BM injection and at days 7 and 46 following Listeria infection by staining with fluorochrome-conjugated anti-CD45.1, anti-CD45.2, anti-TCRβ, anti-CD4, and anti-CD8α antibodies. CD4/CD8 T-cell ratios are depicted (means plus SD; n = 2 to 5). The data are representative of two independent experiments. (C) Relative frequencies of CD4 and CD8 T cells in the spleens of CD45.1^pos wt recipients reconstituted with CD45.2^pos LMP7^−/− plus MECL-1^−/− BM and LMP7^−/− plus MECL-1^−/− recipients reconstituted with wt BM (means plus SD; n = 4).