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T-cell responses in rLM-E1-infected mixed BM-chimeric mice. Mixed BM chimeras were infected with rLM-E1, and the relative frequencies of CD45.1pos and CD45.2pos T cells in the spleens specific for the rLM-E1-derived CD8 T-cell epitope E1B192-200 and CD4 T-cell epitope LLO189-201, were determined by intracellular IFN-γ staining (means plus standard deviations; n = 5). Background, detected in samples incubated without peptide, was subtracted.
