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. 2008 Jan 18;190(6):1891–1899. doi: 10.1128/JB.01452-07

FIG. 2.

FIG. 2.

Transcriptional study of the mpt70/83 locus. (A) Mean luciferase activity, at early exponential phase, of three to five transformants with plasmid coding for luxAB under control of corresponding promoters. The black bars represent the luciferase activities of transformed BCG Russia, whereas the white bars represent those of BCG Pasteur. The dashed line corresponds to minimal level of confidence. (B) Northern blot results of RNA from BCG Russia (R) and BCG Pasteur (P) probed alternatively with mpt70-, dipZ-, or mpt83-specific DNA and detected by ECL methods. The transfer control corresponds to the membrane photographed under UV light. (C) Schematic representation of the results obtained for the mpt70 locus regulation with functional promoters (black arrow), SigK-regulated promoters, and Northern blot-deducted RNA species. Abbreviations: 71, Rv2871; 41, MT2941; 76, Rv2876; 48c, Rv0448c; and 49c, Rv0449c.