TABLE 1.
Oligonucleotide primers used in this study
| Primer name | Sequence (5′→3′)a |
|---|---|
| pRv2871F | CGTTCTAGAGGATGTCGCCGACC |
| pRv2871R | TCGGGATCCGCACGCATGGCATCT |
| pMPB83F | GGGTCTAGAAGATAGCGTGCTCG |
| pMPB83R | CGTGGATCCTCGCTTCGATTCCT |
| pATF | AACTCTAGACTGACGCCAAGCTG |
| pATR | CCGGGATCCGCTTGGGATATCAC |
| pDipZF | TGCTCTAGAACACCGCCGGGCTG |
| pDipZR | GAGGGATCCGACTAGTGCGAGGG |
| pMPB70F | TACTCTAGAGACGTACTTCGGAGT |
| pMPB70R | CCGGGATCCTGTTCTTTACCTTCAT |
| pRv2876F | CAATCTAGAGTTGACAACGCTGAC |
| pRv2876R | GATGGATCCGGTTGGACTGACGTC |
| pMPB53F | CGGTCTAGACGGTGGGTCTGGC |
| pMPB53R | CGGGGATCCCAGGCGAAGACTC |
| pRv0448F | CCATCTAGACCAGGCGCTGCTG |
| pRv0448R | TCGGGATCCACGTACCAGCTGTA |
| pRv0449F | CCATCTAGATTGGCTCGTGCTTGC |
| pRv0449R | CGGGGATCCCACTTCCGACAAC |
| pSigAF | ACATCTAGAGGTCGGCGGCAAG |
| pSigAR | TTTGGATCCTGCCACATACACCCC |
| pHSP60F | CGTTCTAGATTAGCACGTCGATGC |
| pHSP60R | CGGGATCCGCAATTGTCTTGGCCAT |
| Rv0444OF | GTGCTGCAGGGTGCGGCCAACG |
| Rv0444OR | GGAAAGCTTGATAACGGCGACATC |
| SigKflavF | GCAGGTACCCTCCCCCGTGGC |
| SigKflavR | GCAAAGCTTACGGCGTCGCCAG |
| ATSigKflavR | GAAAAGCTTAGTAATGCCCTGCCG |
| EcoRI1 | GCATTTGAGCTCCACGTCAGCGACTG |
| EcoRI2 | AATTCAGUCGCUGACGUGGAG |
| EcoRIF | GAGCTCCACGTCAGCGACTGAA |
| Uni70F | CCCGCGTTCGACAAGCTGCCG |
| Uni70R | CCACCGGAACCTGCGACATTCC |
| dxrUniF | GCTCTACGCCCTTGTTGACCAG |
| Rv2876R | GTGCTCCACCTCGGTACTTG |
a
Underlining indicates differences in primers with respect to the template sequence. Boldface indicates restriction sites.