TABLE 1.
Strains of B. subtilis used in this study
Strain | Genotypea | Phenotype | Source |
---|---|---|---|
CYBS400 | Wild type | Control; prototroph | Our stock |
CYBS318 | CYBS400 Δ(rtpA-ycbK)::Spr | No AT, no YcbK | 29 |
CYBS223 | CYBS400 mtrBΩTcr | Phenylalanine bradytroph, no TRAP | 24 |
CYBS542 | CYBS400 amyE::[P-ΔLR(ΔT) Δ(S/D and AUG for rtpLP-ΔrtpLP ycbK′-′lacZ] Cmr | Phenylalanine bradytroph, overproduction of AT, no YcbK | 8 |
BS166 trpE26 | trpE26 | Tryptophan auxotroph | 7 |
BS166 trpE26 ΔmtrB | BS166 trpE26 mtrBΩTcr | Tryptophan auxotroph, no TRAP | This work |
S/D, Shine-Dalgarno sequence; AUG, start codon.