Skip to main content
. 2008 Jan 18;190(6):1900–1911. doi: 10.1128/JB.01761-07

FIG. 6.

FIG. 6.

EMSAs of the cj0176c-cj0177 intergenic region using Fur. Digoxigenin-labeled DNA was present at 0.0775 nM. Lane 1, no protein, lanes 2 to 5, labeled DNA fragments incubated with 30, 60, 90, and 210 ng/μl C. jejuni Fur, respectively. (a) Labeled probe of the cj0176c side of intergenic region split by PCR to contain cj0176c Fur box (113 bp). (b) Labeled probe of the cj0177 side of intergenic region split by PCR to contain cj0177 Fur box (140 bp). (c) Competitive EMSA. Digoxigenin-labeled intergenic spacer region DNA was present at 0.0775 nM. Lane 1, no protein; lane 2, labeled DNA fragment incubated with 210 ng/μl C. jejuni Fur; lane 3, labeled DNA fragment incubated with Fur (210 ng/μl) with a 1,500-fold increase of competitors (unlabeled intergenic spacer fragment); lane 4, labeled DNA fragment incubated with Fur (210 ng/μl) with a 2,000-fold increase of competitors (unlabeled intergenic spacer fragment).