TABLE 2.
Primers
| Primer function and name | Base sequence (5′-3′)a | Product or annealing site |
|---|---|---|
| Cloning | ||
| 0172cF | GGGGTACCGAGCTATGGCAAGAGGATGTAAG | pCEM1 insert |
| 0172cR | AAAACTGCAGGAGCCTTATGGCATTGATATAG | |
| 0173cF | GGGGTACCGCTTTAATCACTGCTTTAATTACC | pCEM2 insert |
| 0173cR | AAAACTGCAGCATAGCCATGATCACCTGCATTG | |
| 0174cF | GGGGTACCGATAGAGATCAAGCCAGACAAG | pCEM3 insert |
| 0174cR | AAAACTGCAGGTTACCATAATAGCCGTAATGCC | |
| 0177F1 | GGGGTACCGAGAATGTTCAGAGCAAGCAAAAG | pCEM4 insert 1 |
| 0177R1 | TCCCCCGGGCATTAGTAAGTTCATTGGCTTTAATGG | |
| 0177F2 | TCCCCCGGGGTTGTGGTGAATTTAAGCTATGG | pCEM4 insert 2 |
| 0177R2 | AAAACTGCAGCCCACATATCCATTATTTTCAAGTTC | |
| cfrAF | GGGGTACCCATTCGAAGGCTCTTGTATAG | pCEM5 insert |
| cfrAR | AAAACTGCAGCTTCTACCTCCATAAACAAGAC | |
| Inverse PCR | ||
| 0172cInvF | GAAGATCTCCTTGGAAGATTATCGAAATG | pCEM1 |
| 0172cInvR | GAAGATCTCATTGCGCATTTTACAGTAG | |
| 0173cInvF | GAAGATCTCTTAAAATAGGAAATCGCTTTGAG | pCEM2 |
| 0173cInvR | GAAGATCTCTCCTTCTTTGACATGAAGATTG | |
| 0174cInvF | GAAGATCTGATGCAGCTTTGCCTTCCTTG | pCEM3 |
| 0174cInvR | GAAGATCTGTAAAATTGCCCCGAGTTTATAG | |
| cfrAInvF | GAAGATCTTCGCAAGTGGGAGTGGTAG | pCEM5 |
| cfrAInvR | GAAGATCTTCCCAATGGCGCAAACTG | |
| KAR2 complementation | ||
| 0752upF | GGAATAATCAAGCCTACAAAATC | pGEMCWH01 |
| 0752upR | GATTTTGTAGGCTTGATTATTCC | pGEMCWH01 |
| 0752downF | GTCAATCCACAATATACAAGCAAAAC | pGEMCWH01 |
| 0752downR | GTTTTGCTTGTATATTGTGGATTGAC | pGEMCWH01 |
| 0178compF | GGGGTACCCATAGACATTAATTCCTTTTAGATATTTTG | pCEM18 insert |
| 0178compR | AACTGCAGGTATCTGTTGCATTTGCTTCTG | |
| 0177comp InvF | GAAGATCTGCTATGGAGAAATAGATTTAAAAG | pCEM18 |
| 0177comp InvR | GAAGATCTGAATGCTTAAAATAGTATAAAATTTC | |
| Promoter cloning and gel shifts | ||
| 0176c-77 promF | CGGGATCCTAATTCCTTTTAGATATTTTG | pCEM14/15 insert |
| 0176c-77 promR | CGGGATCCTATTATTGTTACTTTCTTAG | |
| 0178promF | CGGGATCCGACATTATATCCTTAAGCCATAAGC | pCEM16 insert |
| 0178promR | CGGGATCCTCCTCCCCCTTATAAATCAAAAC | |
| exbB1promF | CGGGATCCCAAAAATTCCTATTATAGTAGATTTATATTG | pCEM17 insert |
| exbB1promR | CGGGATCCTTGTATATTCCTTAATATTTATATTCAAAAC | |
| 0176c-p-split | TTATTATAATAAAATGAAAATATTTATC | Promoter probes |
| 0177-p-split | CATTTTATTATAATAAATTTTGATAATA |
a
Restriction enzyme sites are shown in bold.