. 2008 Jan 18;190(6):2031–2038. doi: 10.1128/JB.01625-07

TABLE 1.

Strains and plasmids used

Strain or plasmid	Genotype and description	Source and/or reference
B. subtilis strains
168	Wild type, trp	Laboratory stock
PS832	Wild type, trp⁺ revertant of strain 168	Laboratory stock
PS356^a	ΔsspA ΔsspB α⁻β⁻	10
PERM450^a	ΔsspA ΔsspB ΔexoA::tet Δnfo::neo α⁻β⁻ Neo^r Tet^r	20
PERM452^a	ΔexoA::tet Tet^r	20
PERM453^a	Δnfo::neo Neo^r	20
PERM454^a	ΔexoA::tet Δnfo::neo Neo^r Tet^r	20
YB300	recA Cm^r	R. Yasbin (36)
PERM662^a	recA Cm^r	(YB300 → PS832)^b
PERM663^a	recA ΔexoA::tet Cm^r Tet^r	(pPERM374 → PS599)^c
PERM665^a	recA ΔexoA::tet Δnfo::neo Cm^r Tet^r Neo^r	(pPERM337 → PERM663)^c
PS599^a	uvrA42 trp⁺	P. Setlow
PERM515^a	uvrA ΔexoA::tet Tet^r	(pPERM374 → PS599)^c
PERM517^a	uvrA ΔexoA::tet Δnfo::neo Tet^r Neo^r	(pPERM337 → PERM515)^c
Plasmids
pPERM337	1.3-kb SmaI-SmaI fragment containing a Neo^r cassette inserted into the NaeI site of nfo in pPERM282; Amp^r Neo^r	20
pPERM374	469-bp XbaI-BamHI fragment (3′ region of exoA) and 537-bp EcoRI-HindIII fragment (5′ region of exoA) flanking the tetracycline gene of pDG1515; Amp^r Tet^r^d	20

The background for this strain is PS832.

Chromosomal DNA from the strain to the left of the arrow was used to transform the strain to the right of the arrow.

DNA of the plasmid to the left of the arrow was used to transform the strain to the right of the arrow.

See reference 6.