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Purification of rEcxR. E. chaffeensis ecxR was cloned into the pET29a(+) vector, expressed, and purified using nickel chelate chromatography. The purified protein was subjected to 15% SDS-PAGE analysis, followed by Coomassie brilliant blue staining (lane 1) and Western blot analysis using an anti-His tag antibody (lane 2). Lane M contained prestained protein size standards. Each lane contained 1 μg of recombinant protein.
