FIG. 4.

qRT-PCR. Reverse transcription with random hexamer primers generated cDNA using total RNA from the wild type (WT) and SC-E1. Signals from qRT-PCR using specific primers for flaA or flaB were quantified with SYBR green fluorescent dye. The data from one representative experiment are expressed as the average threshold cycles (Ct) for duplicate samples. The experiment was repeated two more times, and similar results were obtained. The enolase gene, eno, was used as a control for data normalization in each experiment.