FIG. 5.

FlaA and FlaB stability. Spectinomycin (100 μg/ml) was added to wild-type or mutant SC-E1 cells to arrest translation. Samples were withdrawn at the indicated time points and analyzed by Western blotting (left panels). Protein density was determined for FlaA (▴) and FlaB (▪) for the wild type (top right panel) and mutant SC-E1 (bottom right panel). The results are expressed as the percent protein density at zero time compared to subsequent time samples. Gels were loaded with 5 μg of lysate of the wild type, 100 μg of SC-E1 for the FlaA blot, and 50 μg of lysate for the FlaB blot. Five micrograms of lysate was loaded for each strain for the anti-DnaK blot.