TABLE 1.
Oligonucleotide primers
| Primer | Descriptiona | Sequence |
|---|---|---|
| P1 | flgE (F) | 5′-CCCCACCCAATTATACTAGC-3′ |
| P2 | flgE (R) | 5′-GTCGCGTCACAAACATTAAG-3′ |
| P3 | kan, EcoRI (F) | 5′-GAATTCGGCGAATGAGCAAGCGCCG-3′ |
| P4 | kan (R) | 5′-ACCGGTGGCGAATGAGCTTGCGCCGTCC-3′ |
| P5 | aadA-1 (F) | 5′-GAATTCGCTACCCGAGCTTCAAGGAA-3′ |
| P6 | aadA-2 (R) | 5′-GAATTCGAAGTCGTCCTCCACGAAGT-3′ |
| P7 | fliF (F) | 5′-GTGGGCAAACTAGAGAGAAT-3′ |
| P8 | fliF (R) | 5′-GCCTCACAGAGTTCTTGACC-3′ |
| P9 | flgE, complement, NdeI (F) | 5′-CATATGATGAGGTCTTTATATTC-3′ |
| P10 | flgE, stop, HindIII-SacI (R) | 5′-GAGCTCAAGCTTATTAATTTTTCAATCTTACAAGTTC-3′ |
| P11 | flgB, promoter, HindIII (F) | 5′-AAGCTTTAATACCCGAGCTTCAAG-3′ |
| P12 | flgB, promoter, NdeI (R) | 5′-CATATGGAAACCTCCCTCAT-3′ |
| P13 | flgE, start (F) | 5′-AGGTCTTTATATTCTGG-3′ |
| P14 | flgE, stop (R) | 5′-GAGCTCAAGCTTATTAATTTTTCAATCTTACAAGTTC-3′ |
| P15 | flaA, RT (F) | 5′-TCATCTGCTATGATTATGCCACC-3′ |
| P16 | flaA, RT (R) | 5′-AGAATAAGCATATTCCATGCCAT-3′ |
| P17 | flaB, RT (F) | 5′-CATATTCAGATGCAGACAGAGG-3′ |
| P18 | flaB, RT (R) | 5′-CCCTGAAAGTGATGCTGGTGTG-3′ |
| P19 | eno, RT (F) | 5′-AACAGGAATTAACGAGGCTG-3′ |
| P20 | eno, RT (R) | 5′-AAATTGCATTAGCACCAAGC-3′ |
a
F, forward; R, reverse; RT, reverse transcription.