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. 2008 Jan 11;190(6):2138–2149. doi: 10.1128/JB.01595-07

FIG. 3.

FIG. 3.

(A) PFGE analysis of VspI-hydrolyzed DNA. The following conditions were used: 14°C, 24 h, 6 V/cm, pulse changing from 10 to 90 s, and 1% agarose. For CMM30-18, the positions of the missing 140-kb fragment and the enlarged 455-kb fragment are indicated by arrows. Additionally, an 8-kb fragment is missing. Lane 1, λ concatemers; lane 2, C. michiganensis subsp. michiganensis NCPPB382; lane 3, CMM30-18. (B) PCR analysis showing the deletion of the chp/tomA region in CMM30-18. PCR was conducted using primers DR1a_up (5′-GTT GCT ACA GCC ATC C-3′) and DR1b_down (5′-GTG CAG CTG CAT GGG TC-3′) and 30 cycles of 1.5 min at 94°C, 1.5 min at 60°C, and 2 min at 72°C. Lane 1, λEcoRI/HindIII; lane 2, C. michiganensis subsp. michiganensis NCPPB382; lane 3, CMM30-18.