TABLE 2.

Regions with low G+C contents and rrn operons in the C. michiganensis subsp. michiganensis chromosome

Region	Position	ORF(s)	Size (kb)	Avg G+C content (%)	Commentsa
chp	38100-117150	CMM_0034-CMM_0077	79.05	64.6	Putative pathogenicity island, 11 pseudogenes; VspI (2×), DraI
tomA	117150-166800	CMM_0078-CMM_0112	49.65	66.8	Putative pathogenicity island
low GC1	193650-196300	CMM_0135, CMM_0136	2.65	55.0
low GC2	306300-310200	CMM_0246	4.1	54.1	Bordered by direct repeat (69 bp)
low GC3	504400-506400		2.1	57.7	VspI
low GC4	552200-556000		3.8	54.9	VspI
low GC5	656600-658600	CMM_0574, CMM_0575	2	61.0	Putative restriction-modification system; bordered by 29-bp direct repeat
low GC6	708300-716650	CMM_0627	8.35	61.1	Putative rhs element, one pseudogene; DraI
low GC7	745100-748700	CMM_661	3.6	53.1	One pseudogene; DraI
low GC8	830520-833300	CMM_0738	2.8	53.3
low GC9	942950-946600	CMM_0828	3.65	58.4	Internal in EPS cluster II; DraI
low GC10	1270450-1274400	CMM_1110	3.95	58.1	Downstream tRNAGln
low GC11	1285200-1286600		1.4	54.7
low GC12	2226650-2228350	CMM_1971	1.7	54.8	Upstream tRNAPro
rrnB	2253517-2247622			54.7	rRNA operon; VspI, DraI
low GC13	2514300-2516050	CMM_2221, CMM_2222	1.75	53.8	Hypothetical pilin gene
low GC14	2587100-2608380	CMM_2286-CMM_2304	21.3	58.9	Putative prophage remnant; bordered by duplicated gene
low GC15	2614420-2615500		1.1	59.9	One pseudogene; bordered by partially duplicated gene
low GC16	2684300-2686750	CMM_2382	2.45	64.8	Putative perforin
rrnA	2873849-2867954			54.7	rRNA operon; VspI, DraI
low GC17	3018000-3019600	CMM_2688	1.6	58.2	Putative acetyl xylan esterase; bordered by 29-bp direct repeat
low GC18	3172450-3174400	CMM_2846, CMM_2847	1.95	56.3	Toxin/antitoxin system
low GC19	3224800-3228500	CMM_2901, CMM_2902	3.7	56.2	Downstream tRNAGly; VspI

^aThe numbers of sites for the rare cutters (VspI and DraI) used in PFGE are indicated.