FIG. 6.
Kinetics of GC-GerU* spore germination in the presence of leucine and proline. Germination rates were calculated from the linear segment of OD changes over time to produce Lineweaver-Burk plots of spore germination at variable leucine concentrations (0.1, 0.175, 0.25, 0.5, 1, 2.5, and 5 mM) and different fixed proline concentrations (0.075, 0.1, 0.15 and 0.2 mM). Apparent Km values ranged from 210 μM to 527 μM in the presence of concentrations of proline that varied from high to low, respectively. Vmax values ranged from 0.052 to 0.041 OD/min for concentrations of proline that varied from high to low, respectively.