TABLE 1.
Strains and plasmids used in this study
| B. megaterium strain or plasmid | Receptor characteristic(s) | Source or reference |
|---|---|---|
| Strains | ||
| PV361 | Nongerminative derivative of QM B1551 | 33 |
| PV361 transformants | ||
| GC-GerU | GerU (containing gerUA, gerUC, and gerUB) | 7 |
| GC-GerU* | GerU* (containing gerUA, gerUC, and gerVB) | 7 |
| GC-OL | GerVB outer loops changed to corresponding GerUB outer loops | This study |
| GC-IL | GerVB inner loops changed to corresponding GerUB inner loops | This study |
| GC-L1 | GerVB with substituted OL1 | This study |
| GC-L2 | GerVB with substituted OL2 | This study |
| GC-L3 | GerVB with substituted OL3 | This study |
| GC-L4 | GerVB with substituted OL4 | This study |
| GC-L5 | GerVB with substituted OL5 | This study |
| GC-L5K334Y | GerVB K334Y | This study |
| GC-L5V335A | GerVB V335A | This study |
| GC-L5E336A | GerVB E336A | This study |
| GC-L5M337K | GerVB M337K | This study |
| GC-GerUloop5 | GerUB Y334 → K337 changed to KVEM | This study |
| GC-GerUVfusion | GerUB TM1 to TM8 fused with GerVB TM9 and TM10 | This study |
| Plasmids | ||
| pGEM-3Z | E. coli cloning vector; Ampra | Promega |
| pHT315 | B. megaterium host plasmid; MLSrb | 2 |
a
Ampr, ampicillin (β-lactam) resistance.
b
MLSr, macrolide-lincosamide-streptogramin B resistance.