TABLE 3.
Strain | Rate of spore germination with indicated germinantb
|
||||
---|---|---|---|---|---|
Buffer | Glucose | Proline | Leucine | KBr | |
GC-GerU | 3 | 85 | 3 | 76 | 10 |
GC-GerU* | 1 | 99 | 100 | 57 | 100 |
GC-IL | 1 | 97 | 100 | 41 | 88 (± 9) |
GC-OL | <1 | 100 | <1 | <1 | 7 |
Spores were germinated in 5 mM Tris-HCl, pH 7.8, for 40 min with 10 mM germinant (50 mM KBr). Spore germination was measured as described in Materials and Methods.
Rates of spore germination are given relative to the A600 loss (65%) for B. megaterium QM B1551 spores in 10 mM glucose, which was set at 100. This value is equivalent to 100% germination after a 40-min incubation. Values are the means of at least duplicate experiments; standard deviation was ≤5% of the mean, except where indicated in parentheses.