TABLE 4.

Rates of germination of spores complemented with GerU* receptors with substitutions to outer loop regions of GerVB^a

Strain	Rates of spore germination with indicated germinantb
	Buffer	Glucose	Proline	Leucine	KBr
GC-GerU*	1	99	100	57	100
GC-L1	4	100	100	71 (± 24)	100
GC-L2	3	100	100	100	10
GC-L3	<1	100	100	85	100
GC-L4	<1	100	100	18 (± 10)	67
GC-L5	2	97	2	10 (± 6)	13

^aSpores were germinated in 5 mM Tris-HCl, pH 7.8, for 40 min with 10 mM germinant (50 mM KBr). Spore germination was measured as described in Materials and Methods.

^bRates of spore germination are given relative to the A₆₀₀ loss (65%) for B. megaterium QM B1551 spores in 10 mM glucose, which was set at 100. This value is equivalent to 100% germination after a 40-min incubation. Values are the means of at least duplicate experiments; standard deviation was ≤5% of the mean, except where indicated in parentheses.