TABLE 5.

Rates of germination of spores complemented with GerU* receptors with single substitutions to putative OL5 residues^a

Strain	Rates of spore germination with indicated germinantb
	Buffer	Glucose	Proline	Leucine	KBr
GC-GerU*	1	99	100	57	100
GC-L5K334Y	2	100	100	63	66
GC-L5V335A	1	97	45 (± 6)	2	41 (± 15)
GC-L5E336A	2	100	1	87 (± 8)	87 (± 12)
GC-L5M337K	<1	99	7	20	15

^aSpores were germinated in 5 mM Tris-HCl, pH 7.8, for 40 min with 10 mM germinant (50 mM KBr). Spore germination was measured as described in Materials and Methods.

^bRates of spore germination are given relative to the A₆₀₀ loss (65%) for B. megaterium QM B1551 spores in 10 mM glucose, which was set at 100. This value is equivalent to 100% germination after a 40-min incubation. Values are the means of at least duplicate experiments; standard deviation was ≤5% of the mean, except where indicated in parentheses.