TABLE 4.
Synergistic effect of various combination of Cel9D with Cel9B, Cel51A, and Cel8B on the hydrolysis of Avicel cellulose
| Cellulase mixturea | Molecular concn (nmol/ml) of enzyme | Reducing sugar produced (μg)
|
DoS | % Conversion | |
|---|---|---|---|---|---|
| Observedb | Theoretical | ||||
| Cel9D | 1.2 | 0.35 ± 0.04 | 1 | 0.07 | |
| Cel9B | 1.8 | 2.18 ± 0.36 | 1 | 0.44 | |
| Cel51A | 1.2 | 1.24 ± 0.18 | 1 | 0.25 | |
| Cel8B | 1.2 | 1.26 ± 0.37 | 1 | 0.25 | |
| Cel9D + Cel9B | 1.2 + 1.8 | 3.00 ± 0.25 | 2.53 | 1.19 | 0.60 |
| Cel9D + Cel51A | 1.2 + 1.2 | 6.36 ± 1.24 | 1.59 | 4.01 | 1.27 |
| Cel9D + Cel8B | 1.2 + 1.2 | 3.71 ± 0.24 | 1.60 | 2.31 | 0.74 |
| Cel9B + Cel51A | 1.8 + 1.2 | 3.61 ± 0.57 | 3.43 | 1.06 | 0.72 |
| Cel9B + Cel8B | 1.8 + 1.2 | 3.27 ± 0.18 | 3.44 | 0.95 | 0.65 |
| Cel51A + Cel8B | 1.2 + 1.2 | 1.93 ± 0.57 | 2.50 | 0.77 | 0.39 |
| Cel9D + Cel9B + Cel51A | 1.2 + 1.8 + 1.2 | 6.30 ± 0.39 | 3.77 | 1.67 | 1.26 |
| Cel9D + Cel9B + Cel8B | 1.2 + 1.8 + 1.2 | 5.34 ± 0.23 | 3.79 | 1.41 | 1.07 |
| Cel9D + Cel51A + Cel8B | 1.2 + 1.2 + 1.2 | 6.62 ± 0.31 | 2.84 | 2.33 | 1.32 |
| Cel51A + Cel8B + Cel9B | 1.2 + 1.2 + 1.8 | 4.27 ± 0.13 | 4.68 | 0.91 | 0.85 |
| Cel9D + Cel9B + Cel51A + Cel8B | 1.2 + 1.8 + 1.2 + 1.2 | 7.20 ± 1.28 | 5.03 | 1.43 | 1.44 |
a
Reaction mixtures (100 μl) were incubated at 37°C for 16 h. Bold indicates a cellulose mixture containing Cel9D.
b
Results are shown as means ± standard deviations.